Plasma atrial natriuretic peptide levels in rabbits with alloxan monohydrate-induced diabetes mellitus


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YEGIN E., AKCAY F., YIGITOGLU M., Celik I. , SUZEK H., ODABASIOGLU F.

JAPANESE HEART JOURNAL, vol.36, no.6, pp.789-795, 1995 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 6
  • Publication Date: 1995
  • Doi Number: 10.1536/ihj.36.789
  • Title of Journal : JAPANESE HEART JOURNAL
  • Page Numbers: pp.789-795

Abstract

The effect of alloxan monohydrate-induced diabetes on the resting plasma atrial natriuretic peptide (ANP) level was investigated in 22 male New Zealand white rabbits. Alloxan monohydrate (100 mg/kg) dissolved in saline at a concentration of 50 mg/ml was administered by a single intravenous injection 3 months before the experimental analysis. The diabetic state was examined 72 h later by quantitative determination of blood glucose levels of >350 mg/dl. Beginning on day 3, 14 animals (Group 1) received a daily subcutaneous injection of 1 U insulin having moderate hyperglycemia (blood glucose concentration [BGC] between 300 and 400 mg/dl). Eight animals (Group 2; normoglycemic controls) received 3.2 U of insulin daily to maintain the BGC below 100 mg/dl. Eight healthy rabbits were included in the study as controls (Group 3). Blood samples for ANP analysis were obtained three months after administration of alloxan monohydrate.