Akademik Veri Yönetim Sistemi
International Biochemistry Congress – 2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Türkiye, 19 - 23 Eylül 2017, cilt.42, sa.1, ss.41
OBJECTIVES: About 50-80% of the fluoride intake in the body is excreted by
kidneys. The present study aimed to investigate the effects of certain minerals (Al,
Se, Mg, Ca) on determination of the impact of NaF on oxidant / antioxidant
capacity and cell viability in renal cell lines which are involved in fluoride
metabolism and are most affected by fluoride excretion.
MATERIALS-METHODS: Cells were propagated with two to three regular
passages per week. Non-toxic mineral doses were determined by the MTT
viability test and NaF IC50 value was identified. Cells were exposed to NaF,
minerals and mineral groups+NaF for 24 hours. Control groups were considered
100% viable and MTT % viability results were determined. Cells were collected
by trypsinization after 24 hours of incubation and analyzed by freeze/thaw
method disintegration. TAS and TOS were determined using commercial kits.
RESULTS: TAS levels were significantly lower in the NaF group compared to
the control (p≤0.05). In Mg and Se plus NaF administered groups, TAS was
slightly lower when compared to the control, albeit a slight increase was observed
(P≤0.05). TOS levels were lower in the NaF administered group when compared
to the control group (p ≤0.05), but it remained the same when minerals were
administered with NaF.
CONCLUSION: The oxidative stress index that increased after NaF
administration in NRK-52E cell line significantly decreased especially in Mg and
Se administered groups, however it was still high when compared to the control.
Keywords: Minerals, renal cell, total antioxidant capacity, NaF, total oxidant
capacity