MOLECULAR APPROACH TO SPECIES IDENTIFICATION IN FISHES: A FOCUS ON Aphanius mento


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Sepil A., Şen F.

in: ADVANCE CONCEPTS ON NATURAL AND AGRICULTURAL SCIENCES, Prof. Dr. Ahmet KAZANKAYA,Assist. Prof. Dr. Mevlüde Alev ATEŞ, Editor, İksad Yayınevi, Ankara, pp.73-94, 2023

  • Publication Type: Book Chapter / Chapter Research Book
  • Publication Date: 2023
  • Publisher: İksad Yayınevi
  • City: Ankara
  • Page Numbers: pp.73-94
  • Editors: Prof. Dr. Ahmet KAZANKAYA,Assist. Prof. Dr. Mevlüde Alev ATEŞ, Editor
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Species determination applications in fishes were generally made by using external morphological features, morphometric and meristic measurements until recent years. However, species identification or affinity relationships of different populations of the same species are carried out at the molecular level in many fishes today. Molecular-based studies allow the systematic identification of fish species to be performed with high reliability using primer pairs on a gene basis. It is a practical and applicable method to determine whether there are intraspecies differences in species with different populations.

 

In previous studies on the subject, DNA is isolated from the blood or tissues of different fish species and identification processes are encountered with primers synthesized. There are molecular-based studies in the determination of species belonging to some families. However, such studies are very limited in species belonging to the Cyprinodontidae family.

 

In this study, it is planned to confirm the place of the species distributed in Nemrut Crater Lake in the systematic classification at the molecular level, which is thought to be Aphanius mento belonging to the family of Cyprinodontidae in terms of external morphological features. In line with the genomic data to be obtained, it is thought to create a database in order to determine the phylogenetic differences between the populations of the species in other regions.