Effect of vitamin E and selenium on necrotic cell death in fluorine toxicity

Usta A., Yüksek V., Çetin S., Dede S.

2nd Eurasia Biochemical Approaches & Technologies (2.EBAT-2019) Congress, Antalya, Türkiye, 26 - 29 Ekim 2019, cilt.1, sa.12, ss.22

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 1
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.22
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Objective: The aim of this study was to investigate the effect of Vitamin E and Selenium on the necrotic pathway against sodium fluoride toxicity in the NRK-52E kidney cell line. Material-Method: Cells were grown in optimal conditions and prepared for analysis. Proliferative concentration of vitamin E and selenium combination, ICF value of NaF was determined by MTT. In this study, control (K), fluorine (F), vitamin E + selenium (E / Se) and fluorine + vitamin E / Se (FES) groups were formed. RNA isolation and cDNA synthesis were performed 24 hours after the application of vitamin E / Se and fluorine to the cells at the specified concentrations. Expression of necrotic genes was determined by RT-PCR. Results: The proliferation enhancing concentration of vitamin E / selenium at 24 hours (Vit E: 60 µM, Se: 0.01 µM) and the IC50 concentration of NaF (3200 µM) were found. In F and FES groups, Ripk1 expression increased by 2.7 and 5 times, Ripk3 increased by 8.3 and 5.1 times, respectively. Conclusion: As a result, it was found that the fluoride given at IC50 concentration affects the necrotic genes studied and the highest increase occurred in Ripk3. It can be concluded that vitamin E + selenium given alone does not alter the genes much, and in the FES group, vitamin E + Se may reduce necroposis in the toxicity caused by fluorine. Keywords: fluorine, cell culture, vitamin E, selenium, necrosis