Akademik Veri Yönetim Sistemi
Eurasian Journal of Veterinary Sciences, cilt.38, sa.4, ss.242-247, 2022 (Hakemli Dergi)
Aim: This study aimed to show the effects of glutathione, recognized by
its antioxidant specialties, on the potential DNA damage (8-hydroxy-2-
deoxyguanosine) and the antioxidant system changes upon its implementation
in BHK-21 cells cultured with high glucose.
Materials and Methods: BHK-21 cell line was regularly surpassed in vitro
conditions (5% FBS, 10% horse serum, 1% L-Glutamine, 1% penicillin/
streptomycin in RPMI 1640 medium, and 5% CO2 and 95% humidity and
37oC) incubated. The control group determined glucose's IC50 value based on
the viability tests executed on MTT cells. Cells were seeded in plates as each
would have 2x106 cells. The control, the test, and the crossbreed test(glucose;
(285 mM), glutathione (250 µM)) groups were prepared. After 24 hours of
incubation, trypsinized cells were designed for analysis through vitrification.
In the lysate of the cell culture that was procured, Oxidative DNA damage, TAS,
TSO, and OSI were measured by the spectrophotometric system with ELISA.
Results: It was observed that 8-OHdG levels increased significantly with glucose
application. Moreover, the increase in the HG+GSH group was more significant when
compared to the control group (p≤0.05). No difference with the control group was
found only in the groupwhereGSHwas applied.As forTAS,whereas any differencewas
observedinGSHusedgroups,theincreaseintheHG+GSHgroupwassignificantcompared
to the control group (p≤0.05). that were the same as the control group. TOS and OSI
considerablyincreasedinHG+GSHimplementedgroupsastothecontrolgroup(p≤0.05).
Conclusion: According to the results, no protective impacts of glutathione at
the cellular level in the doses mentioned above were observed on high-dose
glucose implemented cells. On the other hand, it was revealed that the applied
amounts of glutathione in the process did not cause any toxic effects.