Development of a Method for the Determination of Dyestuffs by Solid Phase Extraction Method


Öter Ç.

INTERNATIONAL CONGRESS ON BIOLOGICAL AND HEALTH SCIENCES, Afyonkarahisar, Türkiye, 26 - 28 Şubat 2021, ss.260

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Afyonkarahisar
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.260
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The increase of environmental problems due to industrial development is a fact. One of the major problems is the existence of dye materials in textile wastewater. Even though the dyes discharged to the receiving environment constitutes only a small portion of water pollution, it is undesirable, especially in developed countries for esthetics and ecology. Also, Dyes may spread toxic and carcinogenic metabolites in anaerobic conditions. Dyes can be treated by physical and chemical methods to prevent pollution in the aquatic environment. In the case of liquid samples, the most commonly used sample pretreatment is analyte extraction using solid phase extraction (SPE) to achieve the required low detection limits. Solid phase extraction (SPE) is a preconcentration or separation technique used in the analysis of complex matrices or low concentration analytes. SPE is based on the transfer of the analyte in the liquid phase to the active areas of the solid phase. SPE is carried out in four stages. In the first step, the solid phase is conditioned with a suitable solvent. With this process, impurities on the solid phase surface are removed. In the second step, the analyte is passed over the solid phase. At this stage, some of the matrix components containing the analyte may be attached. In the third stage, the solid phase is washed with a solvent that has a low elution intensity and allows the matrix components to be removed from the solid phase surface. Finally, the analyte is obtained from the solid phase surface with the help of a solvent that prevents the matrix components from being removed at the same time. In this study, coriander seed was used as a solid phase in the SPE method. Equal concentrations of methylene blue and methyl orange solutions were passed through the conditioned solid phase. After all steps were carried out, the dyestuffs were obtained from the solid phase surface with the help of a suitable solvent. The % recovery value of the obtained solutions was determined using UV spectrometry.