Determination of vitamin D3 effect on DNA damage in sodium fluoride induced kidney cells by comet assay method


Yüksek V., Dede S., Usta A., Çetin S., Taşpınar M.

1st International Veterinary Biochemistry and Clinical Biochemistry Congress (1.IVBCB-2018), Hatay, Turkey, 12 - 15 April 2018, vol.1, no.1, pp.46-47

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Hatay
  • Country: Turkey
  • Page Numbers: pp.46-47
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Aim: This study was planned to demonstrate the effect of sodium fluoride (NaF) administration at different duration (3th hour and 24th hour) and different concentration (IC25, IC50) for each duration on renal DNA damage and in order to the protective effect of vitamin D3 on possible damage. Material and Methods: NRK-52E Cells were replicated in in vitro conditions with regular passages two to three times a week. The MTT viability test revealed values of NaF 3 hours (IC 25: 7750 μM, IC 50: 9600 μM) and 24 hours (IC 25: 1600 μM, IC50: 3200 μM).and proliferation enhancing concentration of vitamin D3 (10 μM). The study groups were identified as control, 3th hour NaF administration (IC25, IC50, IC25 +D3, IC50 +D3) and 24th hour NaF administration (IC25, IC50, IC25 +D3, IC50 +D3). COMET assay (single cell electrophoresis) method was used for DNA damage detection. Results: There was no statistically significant difference in genetic damage index (GDI) changes between hours. However, there was a statistically significant increase (p<0.05) in all study groups according to the control group. No significant difference was found in the effect of vitamin D3 administration on the NaF concentrations applied to the 3th hour. There was a statistically significant decrease (p <0.05) in NaF concentrations applied with vitamin D3 application at 24th hour. Conclusion: As a result, NaF application in cytotoxic concentrations showed genotoxic effect on kidney cell. it was determined that the GDI was the same for both time periods. It was concluded that vitamin D3 may be useful to prevent and correct NaF-induced DNA damage. Keywords: Cell culture, NaF, DNA damage, vitamin D3, comet assay