In vitro Evaluation of The Effect of Vitamin C on Necrosis and Autophagy Caused by Ionizing Radiation


Avrasya Sağlık Bilimleri Dergisi, vol.2, no.4, pp.169-172, 2019 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 2 Issue: 4
  • Publication Date: 2019
  • Journal Name: Avrasya Sağlık Bilimleri Dergisi
  • Page Numbers: pp.169-172
  • Van Yüzüncü Yıl University Affiliated: Yes


ABSTRACT This study was planned to investigate the possible protective effect of Vitamin C on the ionized radiation induced necrotic and autophagicpathways as in vitro. The NRK-52E renal epithelial cells were cultured (RPMI 1640 medium (10% FBS. 1% L-Glutamine (2mM) and 1% Penicillin/Streptomycin). The proliferation-enhancing concentration of Vitamin C (100 µM) was used. In this study 5 groups were designed (Control, Ionizing Radiation, Vitamin C, Ionizing Radiation+Vitamin C, Vitamin C+Ionizing Radiation). The ionizing radiation groups were exposed 8 Gy ionizing radiation. Total RNA and cDNA were isolated from the samples collected from the incubated cells for 24 hours, andexpression levels of target genes were determined by RT-PCR. According to the control gene by RT-PCR; the expression levels of necrotic (RIPK 1, RIPK3) and autophagic (ATG3, ATG5, BECN1, Map3A) target genes were determined. In the radiation group, necrotic and autophagic genes were found to be significantly increased compared to control group. The vitamin C supplementation after radiation decreased genes expression of all genes except Map3A gene. As a result, it was noted that ionizing radiation affects necrotic and autophagic cell pathways in the NRK-52E cell line, and activate cytotoxic mechanism. Vitamin C leads to down regulation for necrotic and autophagic genes, except for Map3A before and after ionized radiation administration. It was considered that the effects of vitamin C on cytotoxicity due to ionizing radiation are worth investigating. Key words: Autophagy, In vivo, Ionizing radiation, Necrosis, Vitamin C