Mitigating roles by Ulexite against acetyleferrocene-induced hematotoxicity, hepatotoxicity, genotoxicity and oxidative stress in Oncorhynchus mykiss

UÇAR A., PARLAK V., Caglar O., Yeltekin A. Ç., ÖZGERİŞ F. B., TÜRKEZ H., ...More

Chemistry and Ecology, vol.40, no.2, pp.118-135, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 2
  • Publication Date: 2024
  • Doi Number: 10.1080/02757540.2023.2297705
  • Journal Name: Chemistry and Ecology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Geobase, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.118-135
  • Keywords: Acetyl ferrocene, genotoxicity, hematological response, hepatotoxicity, oxidative stress, ulexite
  • Van Yüzüncü Yıl University Affiliated: Yes


Acetyl ferrocene (AFC) is being used commonly in several industrial applications and scientific diciplines. Hence, in this study we aimed to determine the LC50 value of AFC, and evaluate the toxicity potential by AFC exposure on rainbow trout (Oncorhynchus mykiss) for 96 h. We also focused to investigate whether UX conferred a protection against AFC-induced toxic insults in fish. For this purpose, some stress related endpoints were measured in blood and liver tissues in a multibiomarker approach. The exposure to AFC observed inhibition/induction of hematological parameters by AFC was slowed down after co-application with UX and AFC. However, UX was found to be ineffective for minimising AFC-induced micronucleus formation after 96 h. Moreover, it was determined that supplementation with UX exhibited activity in favour of antioxidants and inhibited MDA / MPO levels. Again, time-dependent inhibition of Nrf-2 levels, stimulation of IL-6 and TNF-α levels by AFC were ameliorated after co-application with UX. Ultimately, administration with UX suppressed the accumulation of 8-OHdG adducts and caspase-3 levels as compared to only AFC treatment. In a conclusion UX exerted significant protection potency against AFC-induced hematotoxic, oxidative, genotoxic and cytotoxic damages, hence could be a new source of natural protective agents in environment.