In Vitro Cytotoxic Effects And Quality Control Studies On Black Cumin Seed Capsules And Oils In Turkey Market

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Üstündağ B., Derici Eker E., Şahin N. Ö.

Journal of Medical Case Reports and Reviews, sa.2, ss.308-319, 2019 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2019
  • Dergi Adı: Journal of Medical Case Reports and Reviews
  • Derginin Tarandığı İndeksler: Other Indexes
  • Sayfa Sayıları: ss.308-319
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Abstract:
Objective
Black cumin seed is a species of Nigella sativa from the Ranunculacea family. thymoquinone, which ismost important components of black cumin seed oil, is preferred as a traditional source of healingconsidering its potential medicinal properties.
Methods
Black cumin seed oil and soft capsules with different formulations produced by different companies inour country were purchased, quality control tests, in vitro cytotoxicity tests and antioxidant tests wereperformed on these oils and capsules.
Results
The highest amount of thymoquinone was seen in black cumin seed fat 2 (O2) (2.9728 mg / ml), whilethe least amount of thymoquinone was in soft capsule 1 (SC1) group (0.8917 mg / ml). According to thecell viability results obtained by MTT test, O2 experimental group had the highest cytotoxic effect with90.21%, while SC1 experimental group had the lowest cytotoxicity with 94.98%. When the antioxidantvalues were examined, the highest value (913,363
μ
g GAE / mg) was found in fat 1 (O1) and the lowestvalue (180,181
μ
g GAE / mg) was obtained in SC1.
Conclusion
Our study suggests that the cytotoxic effect of black cumin seed can be a promising aspect, especiallyin the development of new treatment methods and anti-cancer drugs.
Keywords:
 black cumin seed oil, soft capsule, cytotoxicity, quality control studies