Determination of MIC Values of Various Antimicrobial Agents and Presence of Resistance Genes in Pasteurella multocida Strains Isolated from Bovine


Gülaydın Ö. , Gürtürk K. , Ekin İ. H. , Öztürk C.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.27, sa.2, ss.217-224, 2021 (SCI Expanded İndekslerine Giren Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Konu: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.9775/kvfd.2020.25093
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Sayfa Sayıları: ss.217-224

Özet

Pasteurella multocida is an important bacterium that can cause respiratory infections in cattle. Due to the usage of antimicrobial agents in

the treatment of the disease frequently, it is critical to follow the antimicrobial susceptibility of the isolates. In this study, minimal inhibitory

concentrations (MIC) of various antimicrobial agents and presence of genes related to resistance were investigated in 59 P. multocida strains

isolated from the respiratory tract of cattle. According to MIC values determined by E-test, all of the isolates were susceptible to enrofl oxacin,

chloramphenicol and gentamicin, but resistant to cefoxitin. In addition, high resistance to ampicillin (88.14%), tilmicosin (64.41%), clindamycin

(83.05%) and streptomycin (59.32%) were observed in the isolates. When the resistance genes were examined by PCR, it was determined that

blaROB-1, tet H, sul II, str A/aphA 1 and erm 42 genes could play an important role in penicillin, tetracycline, sulfamethoxazole + trimethoprime,

aminoglycoside and macrolide resistance, respectively. It was concluded that the usage of ampicillin, tetracycline, sulfamethoxazole +

trimethoprime, macrolide and aminoglycosides should be considered for the treatment of respiratory tract infections caused by P. multocida in

cattle. Also, it was determined that antimicrobial resistance genes could play an important role in the development of resistance in P. multocida.