Fabrication of glucose bioelectrochemical sensor based on Au@Pd core-shell supported by carboxylated graphene oxide

Güler M., Zengin A., Alay M.

Analytical Biochemistry, cilt.667, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 667
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.ab.2023.115091
  • Dergi Adı: Analytical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Au@Pd core-shell, Carboxylated graphene oxide, Glucose oxidase, Biosensor
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The study presents a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on Au@Pd core-shell nanoparticles supported on carboxylated graphene oxide (cGO). The immobilization of GOx was achieved by cross-linking the chitosan biopolymer (CS) including Au@Pd/cGO and glutaraldehyde (GA) on a glassy carbon electrode. The analytical performance of GCE/Au@Pd/cGO-CS/GA/GOx was investigated using amperometry. The biosensor had fast response time (5.2 ± 0.9 s), a satisfactory linear determination range between 2.0 × 10−5 and 4.2 × 10−3 M, and limit of detection of 10.4 μM. The apparent Michaelis-Menten constant (Kapp) was calculated as 3.04 mM. The fabricated biosensor also exhibited good repeatability, reproducibility, and storage stability. No interfering signals from dopamine, uric acid, ascorbic acid, paracetamol, folic acid, mannose, sucrose, and fructose were observed. The large electroactive surface area of carboxylated graphene oxide is a promising candidate for sensor preparation.