Ispec 8th International Conference On Agriculture, Animal Sciences and Rural Development, Ankara, Turkey, 24 - 25 December 2021, pp.3-4
Astragalus L. genus is one of the most challenging genus in terms of identifying in the Flora of Turkey. This genus includes 469 taxa and 217 of these taxa are endemic species. The study was carried out in 2019 to determine the phylogenetic relationships of endemic species belonging to the genus Astragalus L. collected from the Van Lake Basin. 82 taxa belonging to the genus Astragalus L. were collected, 19 of which were found to be endemic while 2 of them were rare. In the study, universal ITS4 and ITS5 primers were used to amplify ITS regions from genomic DNAs isolated from samples. By means of these primers, ITS1, 5.8S and ITS2 regions in rDNA were amplified by PCR. The genome information of the taxa of the genus Astragalus L. whose DNA was sequenced were analysed with different programs (SnapGene, CLC DNA Workbench). Then, DNA sequences obtained from taxa belonging to the genus Astragalus L. were registered in the National Center for Biotechnology Information (NCBI) Genbank and made available to all researchers in the world. In this study, it was also determined that the length of the nrDNA ITS region varied between 669 and 687 base pairs. The results of the study indicated that the most stable secondary structure derived from sequences obtained with the universal primers ITS4 and ITS5 when used in combination with the phylogenetic tree, is an effective tool for the identification of taxa of the genus Astragalus L.