Akademik Veri Yönetim Sistemi
Ispec 8th International Conference On Agriculture, Animal Sciences and Rural Development, Ankara, Türkiye, 24 - 25 Aralık 2021, ss.3-4
Astragalus
L. genus is one of the most challenging genus in terms of identifying in the
Flora of Turkey. This genus includes 469 taxa and 217 of these taxa are endemic
species. The study was carried out in 2019 to determine the phylogenetic
relationships of endemic species belonging to the genus Astragalus L. collected from the Van Lake Basin. 82 taxa belonging
to the genus Astragalus L. were collected,
19 of which were found to be endemic while 2 of them were rare. In the study,
universal ITS4 and ITS5 primers were used to amplify ITS regions from genomic
DNAs isolated from samples. By means of these primers, ITS1, 5.8S and ITS2
regions in rDNA were amplified by PCR. The genome information of the taxa of
the genus Astragalus L. whose DNA was
sequenced were analysed with different programs (SnapGene, CLC DNA Workbench).
Then, DNA sequences obtained from taxa belonging to the genus Astragalus L. were registered in the
National Center for Biotechnology Information (NCBI) Genbank and made available
to all researchers in the world. In this study, it was also determined that the length
of the nrDNA ITS region varied between 669 and 687 base pairs. The
results of the study indicated that the most stable secondary structure derived
from sequences obtained with the universal primers ITS4 and ITS5 when used in
combination with the phylogenetic tree, is an effective tool for the
identification of taxa of the genus Astragalus
L.