Study Objectives: Exercise has a great impact in increases of energy metabolism and imbalance of oxidant-antioxidant status. We aimed to analyse the association between exercise-induced oxidative stress and energy regulatory hormones of leptin, nesfatin-1 and irisin in sedentary male and female subjects. Methods:A total of 30 subjects (15 males and 15 females) performed an aerobic exercise approximately 45 min. Blood samples were taken at baseline and the end of the exercise. ELISA method was used to analyse parameters of leptin nesfatin-1, irisin, total oxidant status (TOS) and total antioxidant status (TAS). Result: Exercise caused a significant increase in levels of irisin and TOS but TAS level decreased. In addition, leptin and irisin levels increased significantly in females, but they did not change significantly in males. Conclusion: However, gender differences have a great impact on energy regulatory hormones which needed to be evaluated in further stud-ies. Energy regulatory hormones did not correlate with the change of TAS and TOS levels.