A STUDY ON DIPLOZOID PARASITES DETECTED IN FISH CAUGHT FROM KARASU STREAM (VAN)

11th INTERNATIONAL ZEUGMA CONFERENCE ON SCIENTIFIC RESEARCH, Gaziantep, Türkiye, 18 - 20 Mart 2024, ss.876-877

Among monogenean parasites, Diplozoids are gill parasites that are more specific to Cyprinidae species.

This group of ectoparasites has an unusual body morphology and a different life cycle. The life cycle

consists of the egg, the free-swimming larva (oncomiracidium - the first invading larval stage), the

second parasitic larva (diporpa) stage, the juvenile (fusion of two diporpas) stage and the final stage,

which is the adult diplozoid (with combined internal systems). Adult diplozoids have a characteristic

structure with four pairs of large hooks in their symmetrical haptors and a pair of median hooks in the

lower part of each haptor, and they have a total X-shaped body appearance. In this study, it was aimed

to determine the diplozoid parasite species that host siraz (Capoeta kosswigi), Tarek (Alburnus

timarensis) and carp (Cyprinus carpio) fish living in Karasu Stream (Van). The gill lamellae of fish

samples obtained in Karasu Stream between April 2023 and February 2024 were examined under a

microscope, and the molecular characterization method was applied to identify the detected diplozoid

parasites. By sanger sequence analysis of PCR amplicons, it was seen that the parasites isolated from

Siraz and Tarek fish were similar to Diplozoon paradoxum and Paradiplozoon homoion species. The

parasite in carp was found to be Diplozoon sp. As a result of the sequence analysis of PCR amplicons,

the diplozoid parasites in Siraz and Tarek fish were the same, and according to the Dendrogram analysis

results, the parasites isolated from Siraz and Tarek fish showed over 98% similarity to D. paradoxum,

while P. homoion and Paradiplozon skrjabini showed 96% similarity. It has been observed that there is

a similarity over. The parasites detected in carp were partially similar to D. paradoxum and P. homoion,

but since they were different from the parasites in siraz and Tarek fish, their diagnosis remained at the

level of Diplozoon sp.