Agar and Glycerol (AG) based Organo-hydrogels containing different cross-linkers and different amounts of Onion Oil were prepared in order to examine the in vitro release of the antibiotic drug Ceftriaxone and the anti-carcinogenic drug Carboplatin. At preparation of the organo-hydrogels was used free radical polymerization technique in the emulsion media. As a control group, Agar-Glycerol (AG) gel and Agar-Glycerol (p (AG-m) and p (AG-g)) hydrogels were synthesized. When balance swelling rates of organo-hydrogels were examined, different swelling behaviors were observed in different solvent environments. When FTIR spectra of gel, hydrogel and organo-hydrogels were examined, it was observed that Onion Oil added different bonds to the structure or deepened existing bonds. It was observed that the drug release amount of Gel, hydrogel and Organo-hydrogels with different properties changed with cross-linker type, ambient pH and drug-polymer interactions. For this purpose, organo-hydrogels were produced containing onion oil in the mass proportion of three different. In order to determine the effect of ambient pH on release rate and amount of drug released, drug release at 4 different pH (at 2.0, 5.5, 7.4 and 8.0) was examined from drug-loaded organo-hydrogels. In organo-hydrogels containing different amounts of onion oil, it was observed that the rate of release changed as the onion oil rate increased. When the amount of the release of p(AG-m-OO) and p(AG-g-OO) organo-hydrogels was compared, it was observed that the rate of release of p AG-g-OO) organo-hydrogels was higher. This is due to electrostatic interactions between the organo-hydrogels synthesized with different crosslinkers and different amounts of onion oil, and the drug. When Ceftriaxone and Carboplatin drug release kinetics were applied, it was found that drug release of organo-hydrogels conformed to Higguci (HM) and Korsmeyer-Peppas (KM) models.