Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and alpha-1 and alpha-2 globulin compared to the control group (p < 0.05), these values were significantly increased (p < 0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The 0- and gamma-globulin concentrations were the lowest in the NaF group statistically (p < 0.05). Despite a significant increase (p < 0.05) in these values in the NaF+Res group, compared to the NaF group, they were still lower compared to the control group. The examination of the percentage of serum protein fractions revealed a reduced albumin in the NaF group compared to the control group but the finding was not statistically significant (p > 0.05). The albumin of the NaF+Res group was statistically higher than that of the control group (p < 0.05). No statistical differences were observed in alpha-1 and alpha-2 globulin across the groups. The 0 -globulin of the NaF group was the highest but not statistically higher than that of the control group. The gamma-globulin percentages in all the groups were found to be lower than the levels in the control group. The albumin/globulin (A/G) ratio decreased in the NaF group but was not significantly different than that of the control group. In conclusion, the alterations in the serum protein fractions due to NaF-induced toxicity, especially the alterations in their concentrations, approached values closer to those of the control group with the administration of resveratrol. We concluded that these results are of potential importance in indicating a favorable role for resveratrol use in preventing and treating fluoride toxicity.