Akademik Veri Yönetim Sistemi
IV-International Conference of Food, Agriculture, and Veterinary Sciences , Van, Türkiye, 27 - 28 Mayıs 2022, ss.5-6
Dodder is a parasitic invasive
plant and poses a significant threat to agricultural production, as it is
difficult to control. Cuscuta campestris
Yunck. is the most widespread species belonging to the genus Cuscuta well as in non-agricultural
areas of farmland in Turkey. In this
study, genetic diversity between 6 different geographical regions of Turkey
from the C. campestris collected from
individuals belonging to 40 populations were studied. Universal ITS4 and ITS5
primers were used to amplify ITS regions from genomic DNAs isolated from these
samples. With the help of these primers, ITS1, 5,8S and ITS2 regions in the
rDNA were amplified by PCR. The genome information of the C. campestris species whose DNA was sequenced was analyzed with
different programs (Geneious Software, CLC DNA workbench and Vector NTI). The
DNA sequences obtained from C. campestris
individuals are registered in the National Center for Biotechnology Information
(NCBI) Gene Bank and made available to all researchers around the world. In
this market, it has been found that the nrDNA ITS region length varies between
570 and 679 base pairs. It has been observed that there is genetic
differentiation in populations resulting from geographical differences between
regions. As a result, to understand the genetic variability of C. campestris between regions, it will
be effective in developing some control strategies to reduce the impact of this
parasitic plant in agricultural areas.