IV-International Conference of Food, Agriculture, and Veterinary Sciences , Van, Turkey, 27 - 28 May 2022, pp.5-6
Dodder is a parasitic invasive plant and poses a significant threat to agricultural production, as it is difficult to control. Cuscuta campestris Yunck. is the most widespread species belonging to the genus Cuscuta well as in non-agricultural areas of farmland in Turkey. In this study, genetic diversity between 6 different geographical regions of Turkey from the C. campestris collected from individuals belonging to 40 populations were studied. Universal ITS4 and ITS5 primers were used to amplify ITS regions from genomic DNAs isolated from these samples. With the help of these primers, ITS1, 5,8S and ITS2 regions in the rDNA were amplified by PCR. The genome information of the C. campestris species whose DNA was sequenced was analyzed with different programs (Geneious Software, CLC DNA workbench and Vector NTI). The DNA sequences obtained from C. campestris individuals are registered in the National Center for Biotechnology Information (NCBI) Gene Bank and made available to all researchers around the world. In this market, it has been found that the nrDNA ITS region length varies between 570 and 679 base pairs. It has been observed that there is genetic differentiation in populations resulting from geographical differences between regions. As a result, to understand the genetic variability of C. campestris between regions, it will be effective in developing some control strategies to reduce the impact of this parasitic plant in agricultural areas.