Antioxidant responses and DNA damage in primary hepatocytes of Van fish (Alburnus tarichi, Guldenstadt 1814) exposed to nonylphenol or octylphenol

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Yeltekin A. Ç., Oğuz A. R.

DRUG AND CHEMICAL TOXICOLOGY, cilt.41, sa.4, ss.415-423, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 4
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/01480545.2018.1461899
  • Dergi Adı: DRUG AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.415-423
  • Anahtar Kelimeler: Oxidative stress, DNA damage, MDA, Van fish, antioxidant, SUPEROXIDE-DISMUTASE, OXIDATIVE STRESS, FREE-RADICALS, 4-NONYLPHENOL, TISSUES, CELLS, ALKYLPHENOLS, GENERATION, INDUCTION, APOPTOSIS
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Alkylphenols, a nonionic surface-active agent group, such as nonylphenol (NP) and octylphenol (OP) are important endocrine-disrupting chemicals (EDC). In this study, the dose- and time-dependent effects of NP and OP were investigated in the primary hepatocyte culture of Van Fish. In this study, samples were taken at different times and biochemical parameters were studied separately. The effects of the chemicals used on SOD, CAT, GSH-Px, MDA, and 8-OHdG were investigated in hepatocyte culture. The antioxidants SOD and CAT were observed to increase in all groups in the primary hepatocyte cultures at the 24th hour after NP and OP administration, whereas the GSH-Px level was observed to increase with OP at the 24th hour and with NP at the 48th hour. The MDA level was observed to reach its highest value for both chemicals in the 24th hour, and the 8-OHdG level was observed to increase toward the end of the follow-up time, compared to the control group (p < 0.05). In conclusion, different doses of NP and OP were found to induce an increase in the levels of antioxidants and the MDA level in Van Fish primary hepatocyte culture. DNA damage, on the other hand, may be considered to appear after longer-term exposure to NP and OP.