Akademik Veri Yönetim Sistemi
JOURNAL OF THE FACULTY OF ENGINEERING AND ARCHITECTURE OF GAZI UNIVERSITY, cilt.30, sa.1, ss.49-56, 2015 (SCI-Expanded)
In this study, enantiomerically pure (S)-1-Phenylethanol was produced with bioreduction of acetophenone that the precursor of many pharmacological product. Derivatives of (S)-1-Phenylethanol (secondary alcohol) is used as the active ingredient of drug of anti alzheimer. Production of (S)-1-Phenylethanol was conducted with the asymmetric reduction reaction of acetophenone that biocatalyst for there action is enzyme of alcohol dehydrogenase (ADH). In this study, a variety of fruits and vegetables were used as source of enzyme (ADH) for reduction of the carbonyl group and ADH enzyme activities were determined of different plants (carrots, potatoes, grapes, radishes) for selection of suitable plant which it has the highest intra cellular enzyme activity. At the end of this election carrot has been considered as a biocatalyst which has highest ADH activity. The effects of size of biyocatalyst, source of enzyme, substrate concentration, temperature, reaction time, pH of there action and the amount of biocatalyst have been investigated on the production of (S)-1-Phenylethanol. It was found that ADH enzyme in fresh carrots catalysed the bioselective asymmetric reduction reactions at high conversion and high enantioselectively (>% 99 ee and 92% c), under the conditions of 2mM substrate concentration, 200 g/L biocatalyst concentration, 1,52 mm biyocatalyst size, reaction temperature 33 degrees C, pH=7 and 150 rpm agitation speed.