Purification and characterization of glucose 6-phosphate dehydrogenase from sheep liver


Turkoglu V. , ALDEMIR S., CIFTCI M.

TURKISH JOURNAL OF CHEMISTRY, vol.27, no.3, pp.395-402, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 3
  • Publication Date: 2003
  • Title of Journal : TURKISH JOURNAL OF CHEMISTRY
  • Page Numbers: pp.395-402

Abstract

Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP(+) oxidoreductase, EC 1.1.1.49; G6PD) was purified from sheep liver by a simple and rapid method. The purification process consisted of two steps: preparation of the homogenate, and 2, 5'-adenosine diphosphate (ADP) Sepharose 4B affinity chromatography.