Purification and characterization of glucose 6-phosphate dehydrogenase from sheep liver


Turkoglu V. , ALDEMIR S., CIFTCI M.

TURKISH JOURNAL OF CHEMISTRY, cilt.27, ss.395-402, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 27 Konu: 3
  • Basım Tarihi: 2003
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.395-402

Özet

Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP(+) oxidoreductase, EC 1.1.1.49; G6PD) was purified from sheep liver by a simple and rapid method. The purification process consisted of two steps: preparation of the homogenate, and 2, 5'-adenosine diphosphate (ADP) Sepharose 4B affinity chromatography.