Advances in nanomaterial based optical biosensing and bioimaging of apoptosis via caspase-3 activity: a review

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Khalilzadeh B., Shadjou N., Kanberoğlu G. S. , Afsharan H., De La Guardia M., Charoudeh H. N. , ...More

MICROCHIMICA ACTA, vol.185, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 185
  • Publication Date: 2018
  • Doi Number: 10.1007/s00604-018-2980-6
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Van Yüzüncü Yıl University Affiliated: Yes


Caspase-3 plays a vital role in intrinsic and extrinsic pathways of programed cell death and in cell proliferation. Its detection is an important tool for early detection of some cancers and apoptosis-related diseases, and for monitoring the efficacy of pharmaceuticals and of chemo- and radiotherapy of cancers. This review (with 72 references) summarizes nanomaterial based methods for signal amplification in optical methods for the determination of caspase-3 activity. Following an introduction into the field, a first large section covers optical assays, with subsections on luminescent and chemiluminescence, fluorometric (including FRET based), and colorimetric assays. Further section summarize methods for bioimaging of caspase-3. A concluding section covers current challenges and future perspectives.