Effects of some drugs on hepatic glucose 6-phosphate dehydrogenase activity in Lake Van Fish (Chalcalburnus Tarischii Pallas, 1811)
JOURNAL OF HAZARDOUS MATERIALS, cilt.143, ss.415-418, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 143
- Basım Tarihi: 2007
- Doi Numarası: 10.1016/j.jhazmat.2006.09.053
- Dergi Adı: JOURNAL OF HAZARDOUS MATERIALS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.415-418
- Anahtar Kelimeler: GOD enzyme, Chalcalburnus Tarischii, inhibition, drug, ERYTHROCYTES IN-VITRO, ENZYME-ACTIVITY, PURIFICATION, ANTIBIOTICS, INHIBITION, LIVER, VIVO
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
Inhibitory effects of some drugs on hepatic glucose 6-phosphate dehydrogenase from Lake Van fish (chalcalburnus tarischii pallas, 1811) were investigated. For this purpose, initially liver glucose 6-phosphate dehydrogenase was purified 899-fold in a yield of 46.24% by using 2',5'-ADP Sepharose 4B affinity gel. In order to control the purification of enzyme was done SDS polyacrylamide gel electrophoresis. SDS polyacrylamide gel electrophoresis showed a single band for enzyme. A constant temperature (+4'C) was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm.