Akademik Veri Yönetim Sistemi
Journal of receptor and signal transduction research, ss.1-14, 2020 (SCI Expanded İndekslerine Giren Dergi)
Bisphenol A (BisPH-A) is a latent danger that threatens our health, which we frequently exposure in
our modern life (e.g. the widespread use of drinking water in plastic pet bottles). But the BisPH-A
induced transient receptor potential melastatin 2 (TRPM2)-mediated oxidative stress and apoptosis in
these cells has not been studied yet. Calcium (Ca2þ) plays an important role in a versatile intracellular
signal transduction that works over a wide range to regulate oxidative stress processes. TRPM2 is activated
by oxidative stress and it has emerged as an important Ca2þ signaling mechanism in a variety
of cells, contributing many cellular functions including cell death. Resveratrol (RESV), which belongs to
the polyphenol group, acts as an antioxidant, eliminating cellular oxidative stress and increasing the
body’s resistance to diseases. The current study aimed to elucidate the effect of antioxidant resveratrol
on TRPM2-mediated oxidative stress induced by BisPH-A exposure in the mouse kidney cortical collecting
duct cells (mpkCCDcl4). The cells were divided into four groups as control, resveratrol (50 mM for
24 h), BisPH-A (100 mM for 24 h) and BisPH-AþRESV. Intracellular free Ca2þ concentrations and TRPM2
channel currents were high in BisPH-A treated cells, but decreased with resveratrol treatment. In addition,
BisPH-A induced mitochondrial membrane depolarization, reactive oxygen species (ROS), caspase
3, caspase 9 and apoptosis values were decreased by the resveratrol treatment. In conclusion, resveratrol
protected cells from BisPH-A induced oxidative damage. In this study, we showed that TRPM2
channel mediates this protective effect of resveratrol.