Akademik Veri Yönetim Sistemi
PAKISTAN VETERINARY JOURNAL, cilt.44, sa.3, ss.924-930, 2024 (SCI-Expanded)
Bovine Viral Diarrhea Virus (BVDV) can evade the immune system by modulating
cytokines production, therefore enabling the virus to establish persistent infections or
exacerbate the severity of disease in infected cattle. The objective of this research was
to investigate interleukins and oxidative stress parameters in cows naturally infected
with BVDV. The study comprised of two groups: a naturally infected group of 15
cattle with clinical signs of BVDV infection or a history of abortion and confirmed
positive for pestivirus by RT-PCR, and a control group of 15 cattle with no abortion
anamnesis or clinical symptoms, confirmed negative by RT-PCR. Anti-inflammatory
cytokines (IL-4, IL-20, IL-10) and pro-inflammatory ones (IL-1, IL-1β, IL-6) values
were measured in all samples using ELISA method. Additionally, the oxidative stress
marker malonaldehyde (MDA) was measured in all samples using the HPLC method.
MDA levels were significantly higher in the infected samples compared with the
controls. While cytokine levels were elevated in the infected group, however, the
differences were not statistically significant. This study found a positive relationship
among anti-inflammatory and pro-inflammatory cytokines, as well as a negative
relationship among IL-1β and IL-1. It was concluded that oxidative stress occurs in
BVDV-infected cattle, and the interleukins measured appear to remain in equilibrium
by inhibiting each other. Notably, IL-20 was measured for the first time in BVDVinfected cattle, making it an important finding. Altogether, it may be concluded on
the basis of these results that cytokines are important in the evaluation of the disease
process