Comparison of Two DNA Barcoding Regions for Molecular Identification of Some Helvella Species from Türkiye

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Terman S., Akçay M. E., Tekpınar A.

MANTAR DERGİSİ, vol.13, no.-, pp.111-118, 2022 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 13 Issue: -
  • Publication Date: 2022
  • Journal Name: MANTAR DERGİSİ
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.111-118
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

In the present study, phylogenetic relationships of some Helvella species from Türkiye (Helvella acetabulum, H. arctoalpina, H. calycina, H. corium, H. crispa, H. elastica, H. lacunosa, H. leucomelaena, H. leucopus, H. solitaria) were assessed using morphological and molecular characters. DNA sequences of internal transcribed spacer (ITS) and translation elongation factor 1-alpha (TEF1-α) were analyzed based on Bayesian Inference methods. Although, the TEF1-α region has less number of indels and consistent nucleotide variations, ITS region can be preferred for figuring out the phylogeny of Helvella species. However, none of the regions may be sufficient alone for the identification of some cryptic and morphospecies within the genu