The anatomic structure and immunoreaction in cells with anti-hFSH and anti-hLH of the pituitary gland in Lake Van fish (Alburnus tarichi Guldenstadt,1814)


Oğuz A. R. , ÜNAL G.

NORTH-WESTERN JOURNAL OF ZOOLOGY, vol.18, no.1, pp.30-34, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 1
  • Publication Date: 2022
  • Journal Name: NORTH-WESTERN JOURNAL OF ZOOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Central & Eastern European Academic Source (CEEAS)
  • Page Numbers: pp.30-34
  • Keywords: Alburnus tarichi, pituitary, histology, histochemistry, Lake Van fish, 2 DISTINCT GONADOTROPINS, IMMUNOCYTOCHEMICAL IDENTIFICATION, ADENOHYPOPHYSEAL CELLS, GLYCOPROTEIN HORMONES, CHALCALBURNUS-TARICHI, LH CELLS, ONTOGENY, EXPRESSION, TELEOSTEI, SUBUNITS

Abstract

The histology of the pituitary gland was investigated in the Lake Van fish (Alburnus tarichi). The adenohypophysis (AH) is composed of the rostral pars distalis (RPD), proximal pars distalis (PPD), and pars intermedia (PI). Seven different cell types were identified in the AH. Most of the cells in the RPD were periodic acid-Schiff (PAS)-negative. In the PPD, polygonal- and ovoid-shaped cells were distinguished, and they were PAS-positive. Polygonal cells were determined to be localized in the dorsal and ventral region of the PPD and around the boundaries of the PI and RPD. These cells showed a strong reaction with anti-human follicle-stimulating hormone (hFSH) and human luteinizing hormone (hLH). Ovoid-shaped cells were observed in the middle region of the PPD, and they showed a weak reaction with anti-hFSH and hLH. The cells localized in the PI were PAS-negative; however, some cells in the follicle surrounding the PI showed a cross-reaction with anti-hFSH and anti-hLH. These cells were more strongly stained with anti- hLH than with anti-hFSH. According to the results, we can conclude that glycoprotein secreting cells are present in all regions of the pituitary, but they are more intensive in the PPD than in the RPD and PI.