Akademik Veri Yönetim Sistemi
FLUORIDE - QUARTERLY REPORTS, cilt.54, sa.4, ss.381-390, 2022 (SCI-Expanded, Scopus)
ABSTRACT: Fluorosis is an important disease both in modern societies as well as in the
previous periods. Paleopathological studies reveal that people in ancient periods
suffered from fluorosis which causes adverse health effects, especially on the
musculoskeletal system as well as soft tissues such as kidneys. In this study, we aimed
to investigate the effects of acute and chronic fluoride administration on some kidney
markers in rats. Fifty-six Wistar albino rats were divided into 7 groups, 8 in each group.
Acute fluoride intoxication was established by administering, in drinking water, 5 ppm
(group 2), 15 ppm (group 3), and 50 ppm (group 4) for 7 days. Chronic fluoride
intoxication was established by administering 5 ppm (group 5), 15 ppm (group 6), and
50 ppm for 90 days (group 7). Control group (group 1) was given tap water. At the end of
the study, the rats were sacrificed under anesthesia and blood samples were taken. The
blood was centrifuged and their serums were separated. CYS-c, KIM-1, and NGAL levels
were measured by ELISA method, and urea, creatinine, total protein, and albumin levels
were measured spectrophotometrically. CYS-c levels were increased in all groups
administered fluoride (p>0.05). Similarly all groups had higher levels of NGAL due to
fluoride exposure and chronic fluoride 5 mg/L group showed significant increase
compared to control (p<0.05). In KIM-1 values, a significant increase occurred in acute
fluoride 15 and 50 mg/L (p<0.05). Significant alterations were also observed in creatinine
and urea values due to fluoride exposure. Consequently, exposure to fluoride may
cause an increase in serum inflammation markers (NGAL, KIM-1) due to differences in
dosage and exposure period. Further long term studies, including molecular and
histopathological assessments, are needed to elucidate the impact of long term
exposure to fluoride on the renal system.