Plum bark necrosis stem pitting associated virus (PBNSPaV) has occurred globally
over the last two decades and is becoming one of the major agricultural issues of
stone fruits. The virus was detected for the first time in plums and has been
reported in the other stone fruit species. The agent, whose dissemination mode is
still unknown, has been first reported in cherries in Turkey grown in Malatya
province. In this study, the K1 isolate of PBNSPaV, identified in a sweet cherry
plant with severe stem pitting and gumming symptoms on its trunk, was partially
characterised. A fragment of Hsp70h gene located on ORF3 of viral genome has
been cloned, sequenced and analysed phylogenetically (Accession number.
FJ231498). The PBNSPaV-K1 isolate showed 93–96% nucleotide sequence
identity to sequences of Italian and American isolates in databases. An RNA
probe has been raised for fast and reliable detection of the agent by molecular
hybridisation. The studies on development of genome specific primers for the
detection of the isolate by one-tube RT-PCR have failed.