In vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum macropodum Boiss. on Cultured Human SH‑SY5Y Neuroblastoma Cells

Çelikezen F. Ç., Türkez H., Fırat M., Arslan M. E., Öner S.

NEUROTOXICITY RESEARCH, cilt.40, sa.1, ss.1-2, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12640-022-00537-z
  • Dergi Adı: NEUROTOXICITY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.1-2
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful

side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important

for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was

carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and

fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase

release (LDH) assays were used to determine the cytotoxic effect of C. macropodum. The extracts were analyzed for

their phenolic content by HPLC–PDA. Major components were determined as 63.600% o-coumaric acid, 15.606% catechine

hydrate, 8.713% rosmarinic acid, 4.376% clorogenic acid, and 3.972% salicylic acid. The obtained results from cytotoxicity

testing revealed that C. macropodum exerted a significant cytotoxic effect on human neuroblastoma cells at all tested

concentrations (p < 0.05). But it did not lead to any cytotoxic potential on human fibroblasts. As a result, the obtained data

clearly revealed C. macropodum exerted a selective cytotoxic action on neuroblastoma cells for the first time.