In this study, the verotoxin types of 73 E. coli strains, 58 animal origin and 15 human origin, were examined by Vero cell culture technique. Typing was performed using Duopath verotoxin GLISA test tool. Of them 67 (91.8%) strains were typed; 31 (42.5%) were VT1, 20 (27.4%) were VT2 and 16 (21.9%) were VT1+VT2. 1 animal origin and 5 human origin totally 6 (8.2%) strains could not be typed. It was seen that this technique used in determining the verotoxin types of human and food origin E. coli strains of can also be used as practical test in determining the verotoxin types of animal origin E. coli strains.