Effect of Fungicide Toxicity on Apoptosis, DNA Damage, and Antioxidant Enzymes in Van Fish

Yeltekin A. Ç.

Pakistan Journal of Zoology, vol.55, no.1, pp.355-360, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.17582/journal.pjz/20210806130845
  • Journal Name: Pakistan Journal of Zoology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Veterinary Science Database
  • Page Numbers: pp.355-360
  • Keywords: Fungicide, Caspase-3, 8-OHdG, Antioxidants, Van fish
  • Van Yüzüncü Yıl University Affiliated: Yes


The extensive use of tebuconazole, an azole group fungicide, causes undesirable toxicity in non-targeted organisms, including fish in aquatic environments. We investigated metabolic responses by observing apoptosis (caspase- 3), DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)), malondialdehyde (MDA), and antioxidants (SOD, CAT, GSH-Px) activity in Van fish kidney and muscle tissue after 24., 48., 72., and 96. h of tebuconazole exposure at a concentration of 2.5 mg/L. The obtained results indicated that caspase-3, 8-OHdG, and MDA levels significantly increased compared to the control (p < 0.05). The kidney and muscle tissues indicated a significant decrease in antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)) compared to the control (p< 0.05). Therefore, these data may reflect one of the molecular pathways that play a role in tebuconazole toxicity.