First Report of Zoonotic Cryptosporidium parvum Subtype IIaA15G2R1 in Dogs in Türkiye

Ayan A., Aslan B., Çelik Ö. Y., Akyıldız G., Orunç Kılınç Ö., Oktay Ayan Ö., ...Daha Fazla

PAKISTAN VETERINARY JOURNAL, cilt.44, sa.4, ss.1263-1268, 2024 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 4
  • Basım Tarihi: 2024
  • Doi Numarası: 10.29261/pakvetj/2024.274
  • Dergi Adı: PAKISTAN VETERINARY JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.1263-1268
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illnessin both humans and animals, leading to acute or chronic diarrhea and even death.The study aimed to investigate the prevalence and subtyping of Cryptosporidiumspp. in shelter dogs in Van province, Türkiye. For microscopic identification of thisparasite, a total of 300 fecal samples were collected and stained with Kinyoun'sacid-fast method. For molecular analysis, the positive samples were subjected toDNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified usingnested PCR. The microscopic examination revealed a 4.67% prevalence ofCryptosporidium spp. Sequence analysis indicated all samples were positive to C.parvum. In addition, GP60 gene was also amplified and C. parvum subtypesIIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequencesof SSU rRNA and GP60 were deposited in GenBank. To our knowledge,Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time indogs in Türkiye. It is recommended to implement control strategies by awarenesscampaign, preventing stray dogs from freely entering public areas, and properdisposal of dog fece