Akademik Veri Yönetim Sistemi
International Society of Academicians, cilt.1, sa.2, ss.96-104, 2018 (Hakemli Dergi)
Tagetes minuta is a plant from Asteraceae family that it is annual, herbaceous plant, erect and
grow to 2 metres. Nowadays it is used for medicinal and aromatic purposes, dried leaves are used in soup.
Also it is used as insecticide and room odor. This study is carried out in Agricultural Biotechnology Lab in
Faculty Of Agriculture of University of Ondokuz Mayıs with the object of the study was to determined the
tissue culture parameters of Tagetes minuta. The seeds that it is parent material for plant tissue culture was
obtained from variety field of application in Department of Field Crops. Firstly matured seeds of Marigold
were applied to surface sterilized and then the seeds that it is sterilized cultured into hormone-free
Murashige ve Skoog (MS) medium in petri. The seeds were keeped dark for 2 days and keeped climate
chamber for 8 days. After that the explants transferred MS medium supplemented with different
concentration of indole acetic acid (IAA) and benzylaminopurine (BAP). Besides that the best medium of
regeneration optimization was determined for plant. The source of explants is Hypocotyl. This study
investigated the ideal explant source and best dose of growth media for the plant. The plant regeneraiton
frequency and explant source was determined in several of plant growth hormone. Results of statistical
analysis showed that different hormone concentrations and sources of explant had effect on regeneraiton of
the plant in vitro condition.