5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Turkey, 23 - 25 November 2022, vol.1, no.74, pp.86
Since the kidneys are the primary excretion site of orally ingested fluoride, the kidneys are exposed to more
flora than other stays organs. In this study, it was aimed to investigate the effect of magnesium application on
IL-1β gene expression as a result of the induction of the kidney cell with sodium fluoride (NaF). Kidney cell
line was grown in RPMI 1640 medium containing 10% FBS, 1% L-Glutamine (2mM) and 1%
penicillin/streptomycin at 37oC, 5% CO2 and 95% humidity. The IC50 dose of NaF to be applied to kidney cells
for 24 hours was 4250 μM and the proliferative dose of Magnesium was determined as 5 μM by MTT test.
The control was divided into 4 groups as NaF (IC50), Mg and NaF+Mg. Substances were applied to cells and
total mRNA isolated. The resulting mRNA were translated into cDNA. Expression levels of target gene (IL1β) were determined by RT-qPCR determined method. It was determined that the IC50 toxic dose of NaF
applied to kidney cells increased approximately 40 times compared to the control gene. As a result, in this
study, it was determined that IL-1β gene became more active and increased in NaF-induced cell death, and
decreased this effect with Mg administration, bringing it closer to the control level.