The effect of magnesium administration on IL-1B gene expression in sodium fluoride-induced kidney cell line


Çetin S., Dede S., Usta A., Yüksek V.

5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Turkey, 23 - 25 November 2022, vol.1, no.74, pp.86

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.86
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Since the kidneys are the primary excretion site of orally ingested fluoride, the kidneys are exposed to more flora than other stays organs. In this study, it was aimed to investigate the effect of magnesium application on IL-1β gene expression as a result of the induction of the kidney cell with sodium fluoride (NaF). Kidney cell line was grown in RPMI 1640 medium containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin/streptomycin at 37oC, 5% CO2 and 95% humidity. The IC50 dose of NaF to be applied to kidney cells for 24 hours was 4250 μM and the proliferative dose of Magnesium was determined as 5 μM by MTT test. The control was divided into 4 groups as NaF (IC50), Mg and NaF+Mg. Substances were applied to cells and total mRNA isolated. The resulting mRNA were translated into cDNA. Expression levels of target gene (IL1β) were determined by RT-qPCR determined method. It was determined that the IC50 toxic dose of NaF applied to kidney cells increased approximately 40 times compared to the control gene. As a result, in this study, it was determined that IL-1β gene became more active and increased in NaF-induced cell death, and decreased this effect with Mg administration, bringing it closer to the control level.