The present study was carried out to investigate whether fresh butter could affect on the serum lipid profile, total triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-c), high density lipoprotein cholesterol (HDL-c), %LDL-c and HDL-c/LDL-c ratio in human. The research was conducted on fifteen volunteer healthy human males, whose background, clinical and dietary information were collected. Fresh butter 20 g/day was taken by individuals at breakfast for 20 days without changing their traditional diet. Blood samples were taken at 10th and 20th days from each individual. According to experimental data, fresh butter consumption was found to cause different effects on the levels of serum TG, TC, LDL-c, HDL-c,%LDL-c and HDL-c/LDL-c in comparing with the beginning values. Their values did not significantly increase at 10th day. However, the levels of TG and HDL-c increased significantly at 20th day of butter consumption period. Also, there was an appreciable increase in TC, LDL-c and %LDL-c levels causing arteriosclerosis at both 10th and 20th day of research period. It was concluded that fresh butter might affect on human lipid profile.