Investigation on the relationship between lymphocyte subsets, immunoglobulin levels and adenosine deaminase activities in immunosuppressive dose methylprednisolone treated dogs

Altug N., Agaoglu Z. T.

BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, vol.51, no.1, pp.109-115, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 1
  • Publication Date: 2007
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.109-115
  • Van Yüzüncü Yıl University Affiliated: No


Seven healthy dogs were administered with methylprednisolone orally (2 mg/kg body weight/d) at the beginning of the study (between 0-10 d), then the dosing was decreased at 10 d intervals throughout 10 to 60 d in an alternate-day therapy programme. Drug administration to the dogs was stopped on the 60 d. Total leukocyte count; polymorphonuclear leukocytes, and neutrophil ratios increased and eosinophil, mononuclear leukocytes, lymphocyte, and monocyte ratios were decrease when these values were compared to changes between days. Furthermore, in lymphocyte subsets, B lymphocytes and CD 8 T lymphocytes ratios were found to decrease. In addition, CD 3 T lymphocyte ratios decreased on the 61 d and CD 4 T lymphocyte ratios increased on the 21, 41, 51 and 61 d. IgM and IgG levels decreased between the days. Serum total ADA and ADA 1 activities decreased during the application, but ADA 2 activity did not change significantly. As a result, after immunosuppressive dose of methylprednisolone application, reduction in the ADA and ADA 1 activity can be used as determinant of the suppression of immune system components.