The determination of serum protein fractions in calves with neonatal diarrhea

Usta A., Çetin S., Yüksek V., Ekici P., Dede S.

1st International GAP agriculture and livestock Congress (1.UGAP-2018), Şanlıurfa, Turkey, 25 - 27 April 2018, vol.1, no.1, pp.448

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Şanlıurfa
  • Country: Turkey
  • Page Numbers: pp.448
  • Van Yüzüncü Yıl University Affiliated: Yes


Abstract Calf diarrhea is one of the important factors causing the serious economic loss in cattle breeding. This study was planned to electrophoretically investigate serum protein fractions in neonatal diarrhea.This study was carried out on 20 calves showing signs of diarrhea at 1-20 days of age in different breeds and sexes in the possession of the grower in the Van region. In addition, 10 healthy calves were used as the control group. Blood samples were taken from the V.jugularis of the animals. The collected serum was separated into protein fractions by cellulose acetate electrophoresis method (Helena Lab-TitanIII Serum Protein). The resulting bands were evaluated in the Platinum 3.0 program. Serum protein concentrations were determined. All results were compared statistically.When the concentrations of serum protein fractions are compared; There was no difference between the groups in terms of total protein, alpha globulins, and gamma globülin levels. Albumin and beta globülin levels were found to be higher in the diarrhea group than in the control group (p≤0.05). There was no difference in terms of albumin, alpha and beta globulin in grams (%)percent in all groups. However, the A/G ratio was found to be higher in the diarrhea group than in the control group (p≤0.05) and gamma globulin level was lower in the diarrhea group (p≤0.01). It was determined that serum protein fractions concentration did not change significantly in the diarrhea group. However, it was determined that the A/G ratio in grams percent increased, gamma globulins were found to be low. Key Words: calf, electrophoresis, neonatal diarrhea, serum proteins, serum protein fractions