The amounts of Fe, Ba, Sr, K, Ca and Ti were determined qualitatively and quantitatively by using energy-dispersive X-ray fluorescence in eight lichen species, Pinus sylvestris, rock and soil in district of Erzurum province (Turkey). Lichen species were Cetraria pinastri (Scop.) Gray, Dermatocarpon miniatum (L.) W. Mann, Lecanora muralis, (Schreb.) Rabenh., Pseudevernia furfuracea (L.) Zopf., Ramalina capitata (Ach.) Nyl., Rhizoplaca chrysoleuca (sm.) Zopf., Rhizoplaca melanophthalma (DC.) Leuckert & Poelt, and Xanthoparmelia somloensis (Gyeln.) Hale. The standard addition method was used for the determination of the concentration of Fe, Ba, Sr, K, Ca and Ti. The experimental setup consists of a Si(Li) detector with a resolution of 147 eV at 5.9 keV and annular Fe-55 and Am-241 radioactive sources. By the use of intersite, interelement and interspecies comparisons, it is concluded that some metals within the thalli of lichens in Erzurum province may reach high levels even when these plants are growing in rural and isolated sites. (C) 2004 Elsevier Ltd. All rights reserved.