A Comprehensive Study on Chemical and Biological Investigation of Thymus Brachychilus Jalas: A Rich Source of Ursolic and Oleanolic Acids

Fırat M.

ANALYTICAL LETTERS, vol.1, no.1, pp.1-11, 2024 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 1 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.1080/00032719.2023.2300737
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1-11
  • Van Yüzüncü Yıl University Affiliated: Yes


growing steadily due to their extensive utilization in traditional medicine,

food industry, and pharmaceutical sector, owing to their abundance

in essential oil and phytochemical content, rendering them

commercially significant species. The current work focuses on conducting

a comprehensive analysis of the ethanol extract and essential

oil derived from the root and aerial portions of Thymus

brachychilus Jalas, an endemic species that has not been previously

investigated. Additionally, a novel GC-MS (gas chromatography–mass

spectrometry) technique was developed to quantify the levels of triterpenoids,

which are frequently found in many plants, particularly

those belonging to the Lamiaceae family. The approach was then

used to assess the triterpenoid content of the species. While the

phenolic content of the species was determined by LC-MS/MS (liquid

chromatography–tandem mass spectrometry), the chemical composition

of triterpenoid, essential oil and flavor (aroma) of the plant was

determined by GC-MS. Eucalyptol was the primary ingredient in both

the essential oil and the flavor, accounting for 11.05% and 12.35%,

respectively. In the DPPH and ABTS radical scavenging techniques,

the root ethanol extract exhibited the greatest antioxidant activity

with IC50 values of 26.70 ± 0.23 and 19.16 ± 0.11 mg/mL, respectively.

There was a high level of urease (59.54 ± 1.67% at 100 mg/mL) inhibitory

activity observed in the root ethanol extract in enzyme inhibition

assays, as well as angiotensin (94.80 ± 0.56%), elastase

(40.19 ± 0.39%), and collagenase (48.26 ± 0.12%) inhibition in the aerial

ethanol extract. Moreover, the MCF-7 cell line for breast cancer

had a strong cytotoxic impact when exposed to the essential oil of

the species (vitality%: 1.45 ± 0.01 at 200 mg/mL. The LC-MS/MS and

GC-MS studies revealed that the roots had a significant concentration

of rosmarinic acid (15,801 mg analyte/g extract), but the aerial

portions had a substantial quantity of ursolic acid (152,374 mg analyte/

g extract). The plant exhibits potential in the food, cosmetic,