A GC–MS method validation for quantitative investigation of some chemical markers in Salvia hypargeia Fisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol

Bakır D., Akdeniz M., Ertaş A., Yılmaz M. A., Yener I., Fırat M., ...More

Journal Of Food Biochemistry, vol.44, no.9, pp.1-19, 2020 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 44 Issue: 9
  • Publication Date: 2020
  • Doi Number: 10.1111/jfbc.13350
  • Journal Name: Journal Of Food Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1-19
  • Van Yüzüncü Yıl University Affiliated: Yes


compounds (sclareolide, sclareol, ferruginol, cryptanol, 6,7-dehydroroyleanone,

suginal, 9,10-dihydro-7,8-dimethyl-2-(1-methylethyl) phenanthren-3-ol, sugiol, inuroyleanone,

12-demethylmulticauline, 7α-hydroxy-β-sitosterol, stigmasterol,

sitosterol, salvigenin, sinensetin, α-amyrin, lupeol, lupenone, 3-acetyl lupeol,

1α,21α-dihydroxy-2,3-(1′1′-dimethyl-dioxymethylene) urs-9(11),12-dien, uvaol,

betulin, pyxinol, lup-(20),29-ene-2α-hydroxy-3β-acetate, betulin 3β, 28β-diacetate,

21α-hydroxy,2α,3β-diacetoxy urs-9(11),12-dien) specific to Turkish Salvia species

was developed and validated. According to the GC–MS analysis results, Salvia hypargeia

Fisch. & C.A. Mey. roots were found to be rich in ferruginol (30787.97 μg/g

extract) and lupenone (23276.21 μg/g extract), and leaves in lupeol (20625.92 μg/g

extract). Additionally, the essential oil and aroma contents of this species were identified

by GC-MS technique. According to the LC-MS/MS results, especially S. hypargeia

leaf extract was rich in rosmarinic acid (38035.7 μg/g extract) and isoquercitrin

(4136.91 μg/g extract). Furthermore, anticholinesterase, antiurease, antityrosinase

and antielastase inhibitory, antioxidant, cytotoxic activities of the ethanol extracts,

essential oil, and major components of the species were evaluated. Antioxidant potentials

of all extracts of this species were quite high in all studied antioxidant methods.

Moreover, butyrylcholinesterase and elastase inhibitory capacities of ferruginol,

the major component of S. hypargeia roots, were notable. For these reasons, this

species has a high potential for food and pharmaceutical industries.

Practical applications

This new GC–MS method was applied to S. hypargeia Fisch. & C.A. Mey. and it indicated

that this species possessed high amount of ferruginol and lupeol, and that this

species could be used for their natural sources. According to the results of the activity

studies (antioxidant, anticholinesterase, tyrosinase, elastase, and cytotoxic), this

method was used to exhibit which compound may be responsible for the activities.

This developed and validated method could be easily applied to determine major/